Hi,
I make a librairies around 6Kb for sequencing.
I shear and size DNA and I add adaptator by ligation. After each step, I do a purification with 1x SPRI AMPure XP beads without particular problem.
After long range PCR, I would purifiy with 1x SPRI AMPure XP beads. But something stop an optimal purification during the elution.
I tested 2 enzyme: Long Amp by NEB and Prime STAR by TAKARA but I have the same problem. The Suppliers tell me there are no incompatibilities between the PCR buffer and AMpure XP beads.
Does anyone ever had this problem ?
Thanks
I make a librairies around 6Kb for sequencing.
I shear and size DNA and I add adaptator by ligation. After each step, I do a purification with 1x SPRI AMPure XP beads without particular problem.
After long range PCR, I would purifiy with 1x SPRI AMPure XP beads. But something stop an optimal purification during the elution.
I tested 2 enzyme: Long Amp by NEB and Prime STAR by TAKARA but I have the same problem. The Suppliers tell me there are no incompatibilities between the PCR buffer and AMpure XP beads.
Does anyone ever had this problem ?
Thanks
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