SEQanswers - View Single Post - Polymerase of choice for generating 16S amplicons?

addyblanch · 08-14-2013, 03:33 AM

Could look at KAPA HI-FI?

http://www.kapabiosystems.com/produc...-hifi-pcr-kits

They have become NGS library prep gold standard very rapidly because of their low error rate and minimal GC bias.

Have a look at the Mike Quail paper below:

http://www.nature.com/nmeth/journal/...meth.1814.html

08-14-2013, 03:33 AM	#2
addyblanch Member Location: Nottingham Join Date: Jul 2012 Posts: 29	Could look at KAPA HI-FI? http://www.kapabiosystems.com/produc...-hifi-pcr-kits They have become NGS library prep gold standard very rapidly because of their low error rate and minimal GC bias. Have a look at the Mike Quail paper below: http://www.nature.com/nmeth/journal/...meth.1814.html