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Old 08-14-2013, 06:08 AM   #3
Location: University of Oklaoma

Join Date: Oct 2012
Posts: 40

Honestly? Fermentas Taq. Nothing special- when you think about it you're generating a 300-500bp fragment, and the likelihood of a misincorporation is low enough that I can live with it. It works well and our libraries usually look very good. Interesting about phusion and the issue of barcode swapping- any specific reference for the higher rate of chimera formation?
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