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Old 03-17-2014, 04:10 PM   #9
Catherine_Burke
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Location: Sydney, Australia

Join Date: Oct 2012
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"is there anything suggesting that hi-fidelity polymerases are more prone to chimera-formation/dropping extension runs?"

The link given above (here again http://link.springer.com/article/10....275-012-2642-z) does suggest that proof reading polymerases have a higher rate of recombination. Also from our own data, we saw much less recombination with regular Taq (we used Qiagen) than with Phusion. This was based on the % reads which had incorrect barcode combinations (i.e. recombined in the pooled enrichment PCR).
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