Hello all. I am trying to use SOAPec KmerFreq_AR to filter and correct my reads before assembly. It seems to work great when the option -s is not set. The -s flag sets the space seed and turns on the spaced kmer option. The issue is that it crashes with "Segmentation Fault". No other error output. Does anyone have any ideas on how to troubleshoot this issue? I have tried joining and posting something on the SOAP-Group, but for some reason the group is not approving my messages. I have seen some posts online with similar problems, but no resolutions. Has anyone got this to work?
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...-
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