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Old 11-02-2017, 05:00 AM   #2
Senior Member
Location: US

Join Date: Dec 2010
Posts: 451

Thanks for the information Joseph,

I would have two questions:
Why should the Smart oligos be 5' biotinylated?
How much of a balanced library do you need to spike in to sequence through the template-switching Cs without running into read quality problems?

Thanks in advance!
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