Curious if anyone has any experience automation platforms and the NexteraXT library preparation kits.
We're looking at several possibilities, including an epMotion 5075 TMX. I've also recently learned a little about the SciClone from Caliper and I've seen mention of some other platforms. Eppendorf has a 5075 with a built in cycler but I don't think I'd trust it for library prep, and have no problem with manual intervention for the thermal cycler steps. I've seen some old threads regarding careful programming to avoid magnetic bead carryover with the epMotion, other than that, seems like information is scarce.
Illumina tech support was surprised I'd want to automate because the XT kit is already so fast, and it is, but I'd rather the robot be pipetting while I write grants, etc. We tend to work in full 96-well plates, too.
Curious to know other's experience, if any, with these automated platforms.
TGIF
docbio
We're looking at several possibilities, including an epMotion 5075 TMX. I've also recently learned a little about the SciClone from Caliper and I've seen mention of some other platforms. Eppendorf has a 5075 with a built in cycler but I don't think I'd trust it for library prep, and have no problem with manual intervention for the thermal cycler steps. I've seen some old threads regarding careful programming to avoid magnetic bead carryover with the epMotion, other than that, seems like information is scarce.
Illumina tech support was surprised I'd want to automate because the XT kit is already so fast, and it is, but I'd rather the robot be pipetting while I write grants, etc. We tend to work in full 96-well plates, too.
Curious to know other's experience, if any, with these automated platforms.
TGIF
docbio
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