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Old 01-05-2017, 04:11 PM   #4
Location: València, Spain

Join Date: Apr 2009
Posts: 48

Hi Ola and wdecoster. Thank you very much for your answer, that was definitively helpful.
In the last point I meant the following: if there is no amplification step, how can I assembly a large region? I mean, how a certain region is read several times in order to obtain overlapping reads that allows the assembly? In other words: I got a 1500X coverage of lambda genome in the burn-in protocol. How I got that coverage? I guess I had at least 1500 times the lambda genome, hadn't I?
The following experiment should be a BAC sequencing protocol. Should I amplify the region of interest in order to get a proper number of copies, shouldn't I?
Thank you very much for your help.
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