Hi All
I need to prepare genomic DNA for maize resequencing.
What kit would you reccomend?
I've read (http://www.biomedcentral.com/1472-6750/11/54) that reduction of plastidal and mitochondrial DNA during isolation is beneficial.
What do you think? Is it worth to use procedure from the article or commercial
Kit would be ok?
Would magnetic-bead technique (invitrogen kit) be better than columns?
I plan to isolate DNa from leaves (most obvious choice I think), but maybe roots (softer tissue) would be better (or introduce bacterial DNA from soil/growth medium)?
Maize genome is big ca. 3Gb and highly repetitive. Would organellar DNA compromise sequencing results?
I need to prepare genomic DNA for maize resequencing.
What kit would you reccomend?
I've read (http://www.biomedcentral.com/1472-6750/11/54) that reduction of plastidal and mitochondrial DNA during isolation is beneficial.
What do you think? Is it worth to use procedure from the article or commercial
Kit would be ok?
Would magnetic-bead technique (invitrogen kit) be better than columns?
I plan to isolate DNa from leaves (most obvious choice I think), but maybe roots (softer tissue) would be better (or introduce bacterial DNA from soil/growth medium)?
Maize genome is big ca. 3Gb and highly repetitive. Would organellar DNA compromise sequencing results?
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