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Old 06-26-2011, 08:09 AM   #2
severin
Genome Informatics Facility
 
Location: Iowa @isugif

Join Date: Sep 2009
Posts: 105
Default Biological questions

A paper should be centered around a biological question that you are interested in or is related to your research or grant. RNA-Seq is just one of many tools for conducting scientific research. The items that must be in a paper are dictated by what you are presenting in your paper. Experimental design, replicates, statistical analyses, table of statistics of your next gen data, the inherent assumptions you are making to simplify the analysis (what do you do with the multiple mapped reads?) the programs you are using to do the analysis (tophat, cufflinks). Pretty much everything that is needed for another group to reproduce your results.
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