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Old 03-10-2012, 04:44 PM   #6
Location: Maryland USA

Join Date: Mar 2012
Posts: 12
Smile Reduced Representation Bisulfite Sequencing

Yes this would be better handled offline or in the Methylation forum.

But I'll take a crack at it anyway here in the 'Introductions' forum for grins.

Due to the polyploidy of many plants doing RRBS is going to be tricky at best. Looking into the literature briefly that references the Meissner Nature paper from 2008, subsequent work was all done on 'mammalian genomes', with a particular emphasis on stem cells and cancer (predictably). The 'tricky' part comes in with the polyploid nature pf plants: until we get Oxford Nanopore 100K reads determining haplotype across the genome of a polyploid organism, much less a quantitative methylation status, that work is just going to have to wait.

Thinking about it further 'someone' could do many many PacBio runs to that same end, yet it would also be fraught with difficulties I can think of off the top of my head, not to mention very expen$ive.

So feel free to contact me directly with further discussion, I look forward to hearing from you.

DaleYuzuki is offline   Reply With Quote