Bioruptor shearing of PCR product

[bernieball]

We are pooling multiple PCR products that range in size from 700 to 2000 bp and would like to fragment them to approx. 450-500 bp before tagging them with barcodes.

Has anyone worked with PCR products vs. genomic DNA in a similar manner?

We will be using a Bioruptor UCD-200.

Thanks for your help!

[jchung]

Bernie,

I'm curious to know how your shearing worked for those mixed amplicon sizes. We are trying to develop a shearing protocol as well for amplicon pools of 300bp - 2kb.

Thanks!