Dear All,
I just made a RRBS library. The problem is that I have a lot of long fragments that is not really good since they probably do not contain CpG rich areas.
This is the profile of the library:
Can anyone help me with what I should do?
It is made from 16 samples pooled together. DNA is from blood with good quality. Msp1 digestion worked.
Please help. Do you believe that i can rescue the library with size selection?
Cheers
Emil
I just made a RRBS library. The problem is that I have a lot of long fragments that is not really good since they probably do not contain CpG rich areas.
This is the profile of the library:
Can anyone help me with what I should do?
It is made from 16 samples pooled together. DNA is from blood with good quality. Msp1 digestion worked.
Please help. Do you believe that i can rescue the library with size selection?
Cheers
Emil
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