How to fix FASTQ files with read length varies errors
0
Dear List,
I am a newbie in NGS analysis, I've got a collection of FASTQ files coming from an Illumina NGS analysis, I tried to upload in GEO and I had this reply from the curator:
These fastq files have "read length varies" errors All reads in a fastq file should be the same length. Read lengths of different size originating from a single lane is an error
So, is there any solution to deal with this? I mean, do I have to trim the fastq's or anything similar? I guess that prior to this I have to use FASTQC in order to see what are the length variations? Do you have any idea?
Thanks in advance
0
Dear List,
I am a newbie in NGS analysis, I've got a collection of FASTQ files coming from an Illumina NGS analysis, I tried to upload in GEO and I had this reply from the curator:
These fastq files have "read length varies" errors All reads in a fastq file should be the same length. Read lengths of different size originating from a single lane is an error
So, is there any solution to deal with this? I mean, do I have to trim the fastq's or anything similar? I guess that prior to this I have to use FASTQC in order to see what are the length variations? Do you have any idea?
Thanks in advance
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