Dear All!
Hello, I am working on RNA sequencing NextSeq500 TrueSeq mRNA LT protocol. I have residual adapters after AmPure purification in 2/8 samples. Should I gel extract my these two or all samples or purify with AmPure beads again?
I am wondering, if I might loose sample with AmPure purification. I used .85X beads ratio already.
Any Recommendations? (Adapter dimer starts at 110bp and my library at 200bp)
Regards
Sahil Gupta
Hello, I am working on RNA sequencing NextSeq500 TrueSeq mRNA LT protocol. I have residual adapters after AmPure purification in 2/8 samples. Should I gel extract my these two or all samples or purify with AmPure beads again?
I am wondering, if I might loose sample with AmPure purification. I used .85X beads ratio already.
Any Recommendations? (Adapter dimer starts at 110bp and my library at 200bp)
Regards
Sahil Gupta
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