Hi,
I used NEBnext ultra II DNA library construct kit for illumina to make ChIP-seq library and bioanalyzer showed there are two peaks: one is around 330bp and the other is 570bp(see the attached file). Input DNA is 1ng and amplification cycle is 15. I used Ampure beads to remove the adaptor after ligation and beads to clean the PCR products. Could anyone give me some insights what causes this pattern. Thanks!
I used NEBnext ultra II DNA library construct kit for illumina to make ChIP-seq library and bioanalyzer showed there are two peaks: one is around 330bp and the other is 570bp(see the attached file). Input DNA is 1ng and amplification cycle is 15. I used Ampure beads to remove the adaptor after ligation and beads to clean the PCR products. Could anyone give me some insights what causes this pattern. Thanks!
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