Dear community,
I am new at RNA seq with Illumina and hope you can help me.
We use the MiSeq System with the TruSeq Stranded mRNA Sample Prep Kit. I do low throughput.
After the amplification step I do a BioAnalyzer analysis to check the sample before pooling.
The BioAnalyzer shows me 2 peaks, one at approximately 90bp and one at 124bp. Any suggestions what this could be? I think its adapter dimers but I really dont know how they occur? Does anyone had that problem previously??
Dont hesitate to ask further questions.
I am new at RNA seq with Illumina and hope you can help me.
We use the MiSeq System with the TruSeq Stranded mRNA Sample Prep Kit. I do low throughput.
After the amplification step I do a BioAnalyzer analysis to check the sample before pooling.
The BioAnalyzer shows me 2 peaks, one at approximately 90bp and one at 124bp. Any suggestions what this could be? I think its adapter dimers but I really dont know how they occur? Does anyone had that problem previously??
Dont hesitate to ask further questions.
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