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Old 07-19-2011, 03:27 PM   #2
Senior Member
Location: Ireland

Join Date: Jan 2009
Posts: 101

Hi Sushma,

I have attached our lab protocol for annealing the individual adapters to create the y-shaped product. It is for home-brew indexed SR adapters but I have also used it for our PE stocks. It is based on the original Illumina patent and Nat Methods 2008 Oct;5(10):887-93 paper. While the working stock is stated as 15 uM (from original patent) - we have been using a 1:2 for our RNAseq or 1:20 dilution of the working stock for our ChIPseq libraries - cuts down the potential for adapter dimers. Best of luck.
Attached Files
File Type: pdf IndexAdaptersStocksProtocol.pdf (136.5 KB, 1740 views)
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