Hi all,
I am still learning towards next generation sequencing and still do not have much experience on the field, so hopefully people are willing to share their experience. I wonder what parameters do people use (with BWA and/or BOWTIE) for different analysis such as ChIP-SEQ, RNA-SEQ, miRNA-SEQ etc... I read somewhere that mapping of data from RNA samples (RNAseq) presents different genome mapping challenges than those from DNA, that means there should be different parameters / options for different mapping analysis. Anybody has any advice?
Thanks,
D.
I am still learning towards next generation sequencing and still do not have much experience on the field, so hopefully people are willing to share their experience. I wonder what parameters do people use (with BWA and/or BOWTIE) for different analysis such as ChIP-SEQ, RNA-SEQ, miRNA-SEQ etc... I read somewhere that mapping of data from RNA samples (RNAseq) presents different genome mapping challenges than those from DNA, that means there should be different parameters / options for different mapping analysis. Anybody has any advice?
Thanks,
D.
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