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Old 03-03-2015, 10:09 AM   #5
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,234
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I can think of three reasons:

1- Pipetting inaccuracy caused by calibration/maintenance issues or operator
2- Presence of viscous material in DNA preps (mostly in non-column based extractions). This would show as streaks in wells of gel. This can be avoided by spinning DNA tube or plate at high speed for 5 min to precipitate non-soluble materials and transferring from top of wells.
3- Proper mixing of DNA with reagents or after dilution
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