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Old 03-03-2015, 11:09 PM   #9
Jafar Jabbari
Location: Melbourne

Join Date: Jan 2013
Posts: 1,234

In this case you need to do clean up only as DNA have already been extracted. Sample loss depends on column specifications but one can maximise recovery by eluting with hot buffer and double elution. In my experience 10-15% loss is normal. In your case it will depend on how strongly DNA is bound to viscous material as well which may go through column. Best approach is to trial with less precious sample by quantifying with dsDNA specific reagents before and after cleans up because dsDNA will contribute to final library.
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