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Old 03-04-2015, 11:57 AM   #11
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Location: Canada

Join Date: Oct 2014
Posts: 3


Did you re-run the undiluted DNA side by side with the diluted DNA as a control?

What do the raw fluorescence readings for controls look like between the two runs?

Assuming you have a homogeneous mixture of DNA with no clumping, try adding additional Qubit standards. I have seen a fair deal of variation in the past so now I run 10 controls (2 x each of 0, 25, 50, 75, and 100 ng) and plot it myself to calculate my DNA concentrations.
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