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Old 03-04-2015, 01:33 PM   #13
Terminator
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Location: Canada

Join Date: Oct 2014
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Quote:
Originally Posted by Myrmex View Post
I did run undiluted controls a couple of times but should start doing it every time. If I run undiluted controls over and over (without changing anything) there variation of maybe around 5–20% or so (which I have no problem with, at least it's qualitatively similar). The larger error comes in when we change the dilution and especially when we concentrate the DNA to a lower volume but then it is more viscous—so I imagine as suggested above that this is a serious source of error.

That is a great idea to do the extra standards across the range and then plot- thanks!
I'm not sure why the Qubit only requires two standards (seems crazy).

I found a post (I don't recall the specific thread) where a user recommended running larger volumes for dilute DNA samples. This may also be worth a shot for reducing variability.

Best of luck!
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