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Old 03-05-2015, 12:56 AM   #15
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,234
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Quote:
Originally Posted by Terminator View Post
I'm not sure why the Qubit only requires two standards (seems crazy).
Linear detection range of PicoGreen is four orders of magnitude in 1 ng/ml to 1000ng/ml DNA concentration. As far as one calibrates fluorometer at 0 and 1000 range there is no need to any other concentration in between or standard curve. It seems to be waste of money and time. With correct calibration one needs only to multiply the fluorescence value in dilution factor to calculate original concentration of DNA sample.
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