View Single Post
Old 03-05-2015, 11:36 AM   #17
Terminator
Junior Member
 
Location: Canada

Join Date: Oct 2014
Posts: 3
Default

Quote:
Originally Posted by nucacidhunter View Post
Linear detection range of PicoGreen is four orders of magnitude in 1 ng/ml to 1000ng/ml DNA concentration. As far as one calibrates fluorometer at 0 and 1000 range there is no need to any other concentration in between or standard curve. It seems to be waste of money and time. With correct calibration one needs only to multiply the fluorescence value in dilution factor to calculate original concentration of DNA sample.
The 500 reaction kit is cheap (60 cents/sample) and it takes a matter of minutes to add a few extra standards. You are correct regarding the linear detection range; however, I think you should review linear regression.
Terminator is offline   Reply With Quote