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Old 10-19-2015, 12:00 AM   #2
Bukowski
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Location: UK

Join Date: Jan 2010
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Targeted coverage would normally imply the coverage you were aiming for with an experiment - i.e. in a perfect experiment with perfect pooling you would achieve this coverage. However you don't get perfect experiments, so you may not achieve it.

Coverage and depth of coverage mean the same thing.

Your coverage is not only related to the number of reads, but the size of the thing you are trying to sequence.

If you have a 100bp region covered by 1 100base read, then the coverage is 1x.
If you have a 200bp region covered by 1 100base read, then the coverage is 0.5x

More coverage is better for most applications, with the possible exception of genome assembly.

Last edited by Bukowski; 10-19-2015 at 12:25 AM.
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