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Old 12-18-2016, 08:08 PM   #1
priyanka_15
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Location: new mexico

Join Date: May 2015
Posts: 17
Default low library yield with Ion AmpliSeq Inherited disease panel

Hi All

I am facing very low yield in library when using 10ng gDNA per pool for Inherited disease panel. I have tried many things like increasing DNA amount, target amplification cycles, ensuring fresh 70% ethanol, drying the beads ( I do 2-3 min. as the place we live is very dry). According to protocol, I should get 300ng/ml-1500ng/ml but I am only getting 132-182ng/ml.

Any ideas or advice?

Thanks
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