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Old 04-14-2017, 08:34 AM   #2
pmiguel
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Location: Purdue University, West Lafayette, Indiana

Join Date: Aug 2008
Posts: 2,317
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Hi Hanco,
There is no single answer to your question. Basically every step in your protocol has the potential to cause library construction to occur sub-optimally or even fail altogether. Also many methods of quantitating the amount of input material will be inaccurate. So the actual amount of material used for library construction may be highly variable.

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Phillip
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