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Old 02-02-2011, 12:20 PM   #2
seb567
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Location: Québec, Canada

Join Date: Jul 2008
Posts: 260
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Quote:
Originally Posted by huma Asif View Post
Dear All,
Can any body help me with any experimental method for determining GC content of a draft and complete bacterial genome beside using bioinformatics tools

Regards
I think the obvious experimental method, in my opinion, is to sequence the DNA and use bioinformatics tools.

In EMBOSS, there is a tool called geecee.

http://emboss.sourceforge.net/apps/r...ps/geecee.html



I created a Python script called gc-percentage.py

http://pastebin.com/raw.php?i=rSJKq0Pa

just save the content to gc-percentage.py and

Code:
./gc-percentage.py genome.fasta > genome.gc
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