I have to do bisulfite sequencing and I have searched from literature that, there's primer already available for the region I am interested. I am wondering how can I got the original genome sequence that the PCR amplified?
One of the region I am interested in is H19 in chromosome 11 in human. PCR primers used for amplifying bisulfite-converted DNA was
F: 5'-TGAGTGTTTTATTTTTAGATGATTTT-3'
R: 5'-ACAATACAAACTCACACATCACAAC-3'
Is there any ways that I can search the original genome sequence (not bisulfite converted DNA sequence) that this PCR amplified?
Thanks
One of the region I am interested in is H19 in chromosome 11 in human. PCR primers used for amplifying bisulfite-converted DNA was
F: 5'-TGAGTGTTTTATTTTTAGATGATTTT-3'
R: 5'-ACAATACAAACTCACACATCACAAC-3'
Is there any ways that I can search the original genome sequence (not bisulfite converted DNA sequence) that this PCR amplified?
Thanks
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