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Old 12-12-2016, 10:54 AM   #1
kgoglin
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Location: San Diego

Join Date: Dec 2014
Posts: 17
Default RNA QC: DNA contamination bad for rRNA depletion/mRNA capture

DNA contamination in an RNA sample can be detrimental to the rRNA depletion or mRNA capture assay prior to RNASeq library prep. We currently use the Bioanalyzer for RNA QC but it is not satisfactory for determining DNA contamination, unless it is obvious high molecular weight DNA. Sometimes an RNA with a good RIN score will still fail rRNA depletion/mRNA capture because there is DNA contamination that was not detected by the Bioanalzyer. Are there other types of RNA QC assays that could help us better determine whether or not we have DNA contamination in our RNA samples?
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