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Old 12-12-2016, 12:10 PM   #3
kgoglin
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Location: San Diego

Join Date: Dec 2014
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Thanks for your response. We are not sure what the downstream sequencing would look like if we performed library prep on one of the DNA contaminated samples. Since the Bioanalzyer profile looked odd, we stopped there and DNAsed the Total RNA and repeated rRNA depletion. I posted below, a rRNA depleted sample from Total RNA before and after DNAse treatment. The profile after DNAse treatment is what we normally see as a "good" profile. The profile in which the Total RNA was not DNase treated, was odd.
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