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Old 09-25-2013, 11:48 PM   #3
visserm
Junior Member
 
Location: South Africa

Join Date: Jun 2011
Posts: 7
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Hi JenMar

I haven't used the Pippin in a while and in the end started using PAGE (I do not have easy access to the machine anymore). In order to avoid selecting libraries without an insert I set the range from 133 to 156. This gave me the correct library size range without any adapter-dimers or primer-dimers. I remember that, on the Pippin, I got very sharp peak around 120 that I didn't collect, so the empty library were definitely there before size selection but not afterwards. A library without an insert is around 118 nt so I hope you didn't include the empty library, but PAGE should definitely clarify that and provide the opportunity to select for the actual library with insert.

All the best
Marike
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