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Old 03-22-2014, 10:37 PM   #1
vanillasky
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Location: Europe

Join Date: Mar 2014
Posts: 42
Default Contaminant in dscDNA sample?

A recent illumina run analysis showed that the majority of our sequences were contaminated with 16S rRNA. Prior to dscDNA we used a rRNA depletion method using the RiboZero Bacteria Magnetic kit. We ran our samples on a Pico Chip and saw our sample had lost the peaks from 16S and 23S so the depletion step works. If contamination occurred it must have been either during dscDNA construction or prior to the illumina run. Could you help me determine which step might have introduced contaminants? In my attachment you will see the dscDNA run on a high sensitivity chip. Does this look fine? Please ignore the empty lanes.
Attached Files
File Type: pdf chip result.pdf (65.4 KB, 18 views)
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