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Old 07-24-2014, 07:02 AM   #1
Location: Europe

Join Date: Mar 2014
Posts: 42
Default CLC-Reads do not map back to contigs

I have been using CLC genomics to try and assemble paired end reads from an illumina run (this is an environmental metagenome sample). The assembly is set up so that at the end, the reads are mapped back to the contigs to see how many matched and how many did not match the contigs. I see from our output that a large number of reads do not match the contigs. Is this indicative of misassembly or what could this indicate? Any input would be most appreciated.

p.s. the trimming was done with an adapter input file to remove any of these present in the sequences. We see only a small percentage having undergone adapter removal following the trimming process. The majority of sequences were just trimmed based on quality score.
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