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Old 11-01-2014, 07:56 PM   #5
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Location: Philadelphia

Join Date: Nov 2012
Posts: 5
Default FFPE - qPCR to check X-linking.

Even if your FFPE DNA is not highly fragmented, you should run some qPCR to check for residual cross-linking via the FFPE process. If doing sureselect or nextera, you'll have a severely less diverse library from FFPE compared to the same amount of non-FFPE DNA.
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