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Old 02-02-2016, 08:42 AM   #1
simon_seq
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Location: Cambridge, UK

Join Date: Aug 2012
Posts: 13
Default Automated primer walking

Hi all,
I'm trying to generate a list of oligonucleotide primers for Sanger sequencing, such that for an arbitrarily large DNA sequence I would get an optimised oligonucleotide every ca. 500 bases. How is this best possible? Solutions I have thought along are:
- BatchPrimer 3 (http://probes.pw.usda.gov/batchprimer3/)
- Querying NCBI Primer-Blast through R => but is there a tool for that..?

Thanks!
Simon
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