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Old 11-10-2016, 01:35 AM   #3
Location: Vienna

Join Date: Feb 2016
Posts: 35

As a side note, the reason why you sequence one sample more than once is that you usually do want to have some replicates, i.e. statistical power in the subsequent analyses. This doesn't apply to every case in NGS science, but it usually does.
For example, if you were to calculate the differentially expressed genes and had just 1 sequencing run per condition, your p-values of the differentially expressed genes would be very high. With 2 replicates per sample, they would be a bit lower and you might have some statistical confirmation that those genes are indeed differentially expressed.

This was just an example, but the concept of replicates is very important in NGS (as it is in many science aspects), because sequencing errors happen!

Last edited by Macspider; 11-10-2016 at 01:37 AM.
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