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Old 11-14-2017, 04:07 AM   #5
Location: London

Join Date: Sep 2013
Posts: 10

Thanks JW. The reason I am asking is I'm interested in the biases introduced by different fragmentation methods. Enzymatic methods, such as restriction endonucleases are a different class.

Essentially what I'm trying to establish, is that are metal cations also used to fragment DNA (I know they are used for RNA). I know the phosphate backbone of DNA/RNA carries a significant negative charge which facilitates this method. I believe RNA is slightly less stable than DNA, and I wondered if the method is also used for fragmenting DNA, but I can't seem to find reference to it online or in sequencing protocols.

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