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Old 05-30-2018, 06:06 AM   #1
seqtechno1
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Location: U.S.

Join Date: May 2017
Posts: 21
Default Thermal cycler with variable temperature lid?

Does this machine exist:, for example, a machine that can change the lid temperature during the course of the reactions? Something tells me that having the lid set to 75C when my reverse transcription reaction is occurring at 45C is not a good idea. In addition, I've been getting lots of adapter dimers and/or technical sequences/artifacts in library prep which I see on the bioanalyzer during my library constructions. Could not having a heat-changing lid be the reason? I am talking about cross all different sample types/library prep kits/depletions.
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