hi austinso,
thanks for the information. i'm going to run through the calculations so that i understand the math better. i will have enough for sequencing because i can normalize all of my libraries to 0.5nM, then just add more volume of these to equilibrate the relative abundances. i've already done those calculations. the ddPCR was just a learning exercise to make sure our primers and probes and everything are working. we got some nice (looking) data out of it, but i haven't analyzed it yet. it looks like 10^6 was the most informative dilution.
