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Old 05-22-2019, 12:47 PM   #2
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Location: Eugene, OR

Join Date: May 2013
Posts: 521

Can you multiplex that much on a Miseq and get enough reads per sample to reconstruct the mitogenome? I guess if the nuclear genome isn't large then a reasonable fraction of reads will go to the mitogenome.

You can use partial reaction of just Nextera DNA Flex and get good results. That might be the cheapest route.
Providing nextRAD genotyping and PacBio sequencing services.
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