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Old 07-12-2019, 03:24 AM   #2
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Location: East Coast USA

Join Date: Feb 2008
Posts: 7,060

The average of raw reads in these two files is 150 bp?
What does that mean? Is the data pretrimmed, which is why you have much shorter reads than expected? If all reads are 150 bp, sequencing facility may have made an error with run setup, if you had requested 2 x 300 bp.
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