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Old 08-05-2019, 05:10 PM   #1
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Location: US

Join Date: Apr 2013
Posts: 222
Default Illumina output questions

Hello, I am gonna do some Illumina 2X300bp amplicon sequencing. I suppose the platform (MiSeq) will run 600 cycles and time fixed. I just recently notice on the official website

It says the running time is 455 hours? It seems to me that the running time range is quite large? What's the difference if you run 4 hours or 55 hours. some questions:

1>Let's both 4 hours and 55 hours will give same length of reads.(In my case is 300bp). However, 55 hours will generate more reads (total number) than 4 hours.

2> Or 4 hours will give you shorter reads (e.g., 100bp) and lower number of reads.

3>Would 4 hours and 55hours running time used same protocols and same amount of regents?

4>Do you know how long normally a sequencing center will run? I would like them to run longer? Would be cost more? How should I tell them? Just simply say run as long as you could?

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