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Old 10-03-2011, 11:53 PM   #1
RCJK
Senior Member
 
Location: Australia

Join Date: May 2009
Posts: 155
Default Amplicon normalization

I came across this this site today:
http://www.axygenbio.com/products/ax...normalizer-kit

And was wondering if anyone has tried this AxyPrep Mag PCR Normalizer kit for normalizing amplicons prior to pooling for sequencing? If so, how'd it go? It seems simple and relatively straightforward, so I'm wondering why I've never heard of it before. (Or any of their other kits for that matter.)

What are people doing anyway to normalize a large number of amplicons prior to pooling and sequencing? Ampure beads/filter plate -> PicoGreen -> dilutions?
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