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Old 01-05-2012, 12:18 AM   #1
visserm
Junior Member
 
Location: South Africa

Join Date: Jun 2011
Posts: 7
Default PippinPrep for TruSeq small RNA library selection

Hi all,

I want to use the PippinPrep for size selection of my TruSeq small RNA libraries. In the TruSeq kit they give the specs for selection an a polyacrylamide gel. They however compensate (according to Illumina) for a ~7 nt deviation in size from what is expected for adapters adding 118 nt. According to them a 21 nt insert fragment will run at ~146 on a PAA gel. Is there somebody who has successfully used the PippinPrep (3% cassettes) and will be able so tell me with the settings they used to select the fragment containing the 21 and 24 nt inserts?

Thanks
Marike
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