I have searched the forum without success so apologies in advance if I have missed this elsewhere.
We are sequencing pooled Nextera XT preps of bacterial genomes, using the bead normalisation. Until now we have not carried out any post-normalisation quantitation and have been reasonably happy with clustering on the MiSeq V3 of between 1000K and 1600K/mm2
Recently, we have had a couple of failed runs with low clustering (<300K), possibly as a result of changes in staffing. We would like to use the Qubit ssDNA kit to quantify the pooled libraries. Does anyone have experience/suggestions/protocols?
We are sequencing pooled Nextera XT preps of bacterial genomes, using the bead normalisation. Until now we have not carried out any post-normalisation quantitation and have been reasonably happy with clustering on the MiSeq V3 of between 1000K and 1600K/mm2
Recently, we have had a couple of failed runs with low clustering (<300K), possibly as a result of changes in staffing. We would like to use the Qubit ssDNA kit to quantify the pooled libraries. Does anyone have experience/suggestions/protocols?
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