Originally posted by kmcarr
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"The RTA version used to basecall your sequence was version 1.8.70.0. Pipeline software version 1.4 was last used in October 2009."
They think that it might be due to library preparation and the fragmentase used to fragment the sample DNA. Anyhow, I trimmed those first few bases away which improved subsequent mapping, and glad to see that it is not out of the norm!
Thanks,
Laura
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