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Old 07-27-2017, 07:53 AM   #9
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Location: Bethesda MD

Join Date: Oct 2009
Posts: 502

Yes, the longer oligos are more expensive. But with dual indexing, 20 primers can accommodate 100 samples.

One more point to consider: PCR-free ligation prep requires a substantial amount (1-2 micrograms) of input DNA so, to generate that amount of amplicon in one round of PCR, you may be introducing the bias that you're trying to avoid.
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