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Old 07-27-2017, 08:50 AM   #10
GMDickson
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Location: UK

Join Date: Feb 2014
Posts: 9
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Quote:
Originally Posted by HESmith View Post

One more point to consider: PCR-free ligation prep requires a substantial amount (1-2 micrograms) of input DNA so, to generate that amount of amplicon in one round of PCR, you may be introducing the bias that you're trying to avoid.
I know I am worried about that as well but not sure how to avoid it. All of the samples will be of the similar but I am trying to work out how much product we are getting out from the first PCR at the moment to see how much of an issue this is.

Many, many thanks for your help. I am sure I will be back with more ponderings (and any results I have) shortly.

Cheers.
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